Carlos González attended to the NuGOWeek in Newcastle (UK)
He presented his on going work as a poster
NuGOweek 2018 is focused on Mitochondria, Nutrition and Health. The Congress is held in the International Centre for Life from Newcastle Upon Tyne (United Kingdom).
Carlos González, a PhD in our research group, presented his ongoing work regarding Grape-seed proanthocyanidins effects on intestinal permeabilization and systemic endotoxemia induced by cafeteria diet in Wistar rats.
The barrier formed by the intestinal epithelium prevents pathogen invasion and translocation of harmful substances and microbial components across the intestinal wall to the blood. Consumption of high-fat/high-sugar diets has been associated with loss of intestinal integrity and increased paracellular transport of endotoxins. In turn, this may induce low-grade systemic inflammation and seems to be a major factor in metabolic alterations underlying chronic diseases such as obesity. Dietary proanthocyanidins are flavonoids that reach the intestine in substantial amounts. These natural compounds are shown to exert a protective effect against obesity-associated intestinal impairment in response to a nutritional challenge. We aim to evaluate the effectiveness of both preventive and corrective grape-seed proanthocyanidin (GSP) treatments ameliorating the intestinal permeabilization and endotoxemia induced by a high-fat/high-sugar diet (cafeteria diet) in Wistar female rats. Seven-week rats were distributed into six experimental groups: One group (STD group) was fed ad libitum a standard chow diet for the whole 17-week experiment. A second group of rats received a cafeteria diet (CAF group) over the same time period. A third group (PRE-CAF group) received a daily preventive treatment (500 mg GSP/kg bw) for ten days and a cafeteria diet for the next 17 weeks. A fourth group was fed with cafeteria diet simultaneously with GSP (500 mg/kg bw) every other week (Simultaneous-Intermittent-Treatment-CAF; SIT-CAF) for 17 weeks. Another two groups (LCD-CAF and HCD-CAF) received both low (100 mg/Kg bw) and high (500 mg/Kg bw) corrective GSP doses daily during the last 15 days. The ovalbumin (OVA) intestinal permeability test was performed at weeks 12 and 17 in all the groups, as well as lipopolysaccharide (LPS) determination in plasma samples. At week 12, OVA and LPS concentrations in plasma were significantly higher on CAF group compared to STD (2- and 4-fold increase, respectively), whereas PRE-CAF and SIT-CAF groups did not show any increase on OVA levels. At 17 weeks, SIT-CAF and STD groups showed comparable OVA and LPS levels in plasma. On the other hand, LCD and HCD treatments were effective in correcting CAF-induced alterations on intestinal permeability, thus reducing plasma LPS to basal levels. These results support the accumulated evidence about the protective effect of proanthocyanidins against the alterations of intestinal barrier produced by an obesogenic diet.